上海滬鼎生物科技有限公司是國內ELISA試劑盒優(yōu)質供應商，代理銷售不同ELISA試劑盒品牌的進口/國產(chǎn)ELISA試劑盒，專業(yè)供應科研實驗所需的培養(yǎng)基，抗體，動物血清血漿，標準品對照品，化學試劑，酶聯(lián)免疫試劑盒，白介素試劑盒，金標檢測試劑盒，微生物，蛋白質，ELISA種屬涵蓋廣，憑借多年行業(yè)經(jīng)驗，完善的售后服務，高質量的產(chǎn)品。

IF=3.6

【文獻標題】Effect of erythropoietin combined with hypothermia on serum tau protein levels and neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy

【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al

【作者單位】邯鄲市邯鄲婦幼保健院（Handan Maternal and Child Health Care Hospital, Handan）

【文獻中引用產(chǎn)品】

人Tau蛋白(TAU)ELISA試劑盒

【關鍵詞】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration

【DOI】doi.org/10.4103/1673-5374.217338

【出版期刊】《NEURAL REGENERATION RESEARCH》

【產(chǎn)品原文引用】

Serum tau protein measurement

Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) and 4, 8, and 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, and then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube and placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science and Technology Co., Ltd.

IF=7.2

【文獻標題】Microcystin-LR influences the in vitro oocyte maturation of zebrafish by activating the MAPK pathway

【作者】Wanjing Liu, Chunhua Zhan, Tongzhou Zhang,et.al

【作者單位】華中農業(yè)大學（Huazhong Agricultural University）

【文獻中引用產(chǎn)品】

斑馬魚卵黃原蛋白（VTG）ELISA試劑盒

【關鍵詞】Microcystin，Meiosis，Oocyte maturation，Hyper-phosphorylation，MAPK

【DOI】doi.org/10.1016/j.aquatox.2019.105261

【出版期刊】《Aquatic Toxicology》

【產(chǎn)品原文引用】

PP2A and MPF enzymatic activity and VTG content

PP2A enzymatic activity was assayed according to the instruction of Serine/Threonine Phosphatase Assay System (Promega, V2460;Madison, Wisconsin, USA). Live oocytes were rinsed and homogenized

in ice-cold PSB to obtain homogenate. The homogenate was added into the provided spin columns with Sephadex beads to remove endogenous phosphates. The supernatant was taken for detecting total soluble protein concentration using a bicinchoninic acid assay (BCA kit;Beyotime, Shanghai,China). The reaction was initiated by adding 15 μL of reaction premixes containing 5 μL of 1 mM phosphopeptide and 10 μL of PPase-2A 5×reaction buffer. After reaction at 37 °C for 30 min,50 μL of stop solution was added to each well. The data was read at 630 nm. The activity levels of MPF and the contents of VTG were determined through the zebrafish MPF ELISA Kit (Mlbio, Shanghai, China) and the zebrafish VTG ELISA Kit (Huding, Shanghai, China), following the manufacturer’s protocol.

IF=9.5

【文獻標題】Alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function in diabetic rabbits

【作者】Xiaowei Zhang,Zhiwei Zhang,Yajuan Yang,et.al

【作者單位】天津醫(yī)科大學第二醫(yī)院(Second Hospital of Tianjin Medical University)

【文獻中引用產(chǎn)品】

兔胰高血糖素（Glucagon）ELISA試劑盒

兔8羥基脫氧鳥苷（8-OHdG）ELISA試劑盒

【關鍵詞】Dipeptidyl peptidase-4 inhibitors, Diabetic cardiomyopathy, Diabetes mellitus, Mitochondrial function, Mitochondrial biogenesis

【DOI】doi.org/10.1186/s12933-018-0803-z

【出版期刊】《BMC BIOLOGY》

【產(chǎn)品原文引用】

Serum biochemical, infammatory oxidative stress measurements 

Blood samples collected were used for serum biochemical examination, including fasting glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-c), high density lipoprotein cholesterol (HDL-c) and creatinine level by full automatic biochemical analyzer. Fasting insulin and GLP-1 level were assessed using Rabbit Insulin ELISA Kit (Wuhan Huamei Biological Engineering Co., Ltd, Hubei Province, China) and Rabbit Glucagon Like Peptide-1 ELISA Kit (Shanghai Huding Biological Technology Co., Shanghai, China) according to the manufacturer’s instructions respectively. Serum oxidative stress related markers Lipid Peroxidation Malondialdehyde (MDA) and 8-hydroxy-2 deoxyguanosine (8-OHdG) were assessed by MDA Assay Kit (Nanjing Jianchen Bioengineering Institute, Jiangsu Province, China) and Rabbit 8-OHdG ELISA Kit (Shanghai Huding Biological Technology Co., Shanghai, China). Serum antioxidant enzyme superoxide Jianchen Bioengineering Institute, Jiangsu Province, China). And the infammation maker high-sensitivity C-reactive protein (hs-CRP) level was detected using Rabbit hs-CRP ELISA KIT (Wuhan Huamei Biological Engineering Co., Ltd, Hubei Province, China).

IF=5.9

【文獻標題】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats

【作者】Rong He，Yujiao Wang，Yijie Yang，et.al

【作者單位】南京財經(jīng)大學（Nanjing University of Finance and Economics）

【文獻中引用產(chǎn)品】

人白細胞介素6(IL-6)ELISA試劑盒

人腫瘤壞死因子α(TNF-α)ELISA試劑盒

人誘導型一氧化氮合成酶(iNOS)ELISA試劑盒

【關鍵詞】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress

【DOI】doi.org/10.1016/j.jff.2019.02.031

【出版期刊】《Journal of Functional Foods》

【產(chǎn)品原文引用】

Materials and reagents

RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).